Shotgun engineering fires up mammalian metabolic design

1. Trolle, J. et al. Nat. Biotechnol. https://doi.org/10.1038/s41587-026-03318-7 (2026).

   Article  Google Scholar 

2. Petzold, C. J., Chan, L. J. G., Nhan, M. & Adams, P. D. Front. Bioeng. Biotechnol. 3, 135 (2015).

   Article  PubMed  PubMed Central  Google Scholar 

3. Liberante, F. G. & Ellis, T. Curr. Opin. Syst. Biol. 25, 1–10 (2021).

   Article  CAS  Google Scholar 

4. Rai, K. & O’Connell, R. W. et al. Nature 650, 1035–1044 (2026).

   Article  CAS  PubMed  Google Scholar 

5. Payne, S. H. & Loomis, W. F. Eukaryot. Cell 5, 272–276 (2006).

   Article  CAS  PubMed  PubMed Central  Google Scholar 

6. Trolle, J. et al. eLife 11, e72847 (2022).

   Article  CAS  PubMed  PubMed Central  Google Scholar 

7. Rai, K., Wang, Y., O’Connell, R. W., Patel, A. B. & Bashor, C. J. Curr. Opin. Biomed. Eng. 31, 100553 (2024).

   Article  CAS  Google Scholar 

8. Zhao, Y. et al. Nat. Biotechnol. 42, 1693–1704 (2024).

   Article  CAS  PubMed  Google Scholar 

9. Wang, N. B., Beitz, A. M. & Galloway, K. E. Curr. Opin. Syst. Biol. 24, 18–31 (2020).

   Article  PubMed  PubMed Central  Google Scholar 

10. Brixi, G. et al. Nature 652, 1349–1361 (2026).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Author notes

1. Kshitij Rai

   Present address: Department of Genome Sciences, University of Washington, Seattle, WA, USA

Authors and Affiliations

1. Department of Bioengineering, Rice University, Houston, TX, USA

   Kshitij Rai & Caleb J. Bashor

2. Graduate Program in Systems, Synthetic and Physical Biology, Rice University, Houston, TX, USA

   Kshitij Rai

3. Department of Biosciences, Rice University, Houston, TX, USA

   Caleb J. Bashor

4. Rice Synthetic Biology Institute, Rice University, Houston, TX, USA

   Caleb J. Bashor

Corresponding author

Correspondence to Caleb J. Bashor.