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  • High-throughput metabolic engineering in mammalian cells

    High-throughput metabolic engineering in mammalian cells

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    Nature Biotechnology (2026) Cite this article

    Engineering complex phenotypes into mammalian cells holds profound potential but remains a largely intractable engineering challenge. We developed shotgun genetic engineering (SGE), a multiplexed strategy that screens millions of combinatorial solutions in a single experiment. This approach uses selection strategies that reveal effective designs, facilitating rapid engineering of complex biological functions.

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    Fig. 1: Highly multiplexed exploration of combinatorial design spaces with SGE.

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    References

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    Trolle, J. et al. Highly multiplexed mammalian metabolic engineering with a shotgun approach. Nat. Biotechnol. https://doi.org/10.1038/s41587-026-03318-7 (2026).

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    High-throughput metabolic engineering in mammalian cells. Nat Biotechnol (2026). https://doi.org/10.1038/s41587-026-03317-8

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    • DOI: https://doi.org/10.1038/s41587-026-03317-8